Insulin

Charisela Rapid Insulin Assay

Features rapid results and a simple protocol

Catalog number Number of Assays
40-1000 100
45-1000 500

The Charisela quantitative insulin assay is a bead-based fluorescent flow cytometry assay specifically designed to expedite islet research by replacing traditional ELISA assays.

The assay features:
  1. Samples ready for analysis in about 1 hour
  2. Quantitation of high concentration insulin levels without dilution
  3. Simple protocol with 1 wash step
  4. Wide dynamic range of human insulin
  5. Only 5 components with 1x working solutions for your convenience
For human, primate, porcine and rodent insulins.
Assay range:  1 mU/mL – 1,000 mU/mL


Calibration curve



 Insulin  Concentration (mU/mL) Ave
N=3		 Std. Dev. CV%
3.2 162.5 1.14 0.7
25 105.8 1.17 1.0
50 73.7 2.45 3.3
100 40.4 2.84 7.0
200 16.9 1.24 7.3


Within run precison

Insulin Concentration (mU/mL) Ave
N=3		 Std. Dev. CV%
0.8 171.9 2.0 1.1
1.6 168.3 1.3 0.8
3.2 165.9 1.6 1.0
6.3 152.7 1.0 0.6
12.5 138.9 1.6 1.1
25 105.1 1.3 1.2
50 71.9 7.1 9.9
100 41.1 2.3 5.5
200 20.6 3.2 15.7


Recovery
Expected Insulin Concentrations (mU/mL) Calibrator Median
N=3		 Standard Median
N=3		 Actual standard
(mIU/mL)		 Recovery %
0 171.6 171.6    
3.2 161.9 162.7 3.0 93
6.3 146.3 150.7 5.3 84
25 101.8 93.5 27.9 112
50 52.8 59.6 44.7 89
100 32.1 36.9 79.1 79

Insulin Table

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FOR RESEARCH ONLY